Indirect ELISAs identify antibodies with the appropriate specificity by immobilizing the antigen on a surface and incubating it with a hybridoma cell supernatant. Researchers also use techniques ...
Then the fun starts. Some labs will use the standard polyclonal and hybridoma techniques, while others will apply various alternative approaches. At the end of the exercise, it will be possible to ...
Screening a hybridoma fusion and then subcloning candidate ... J.F. A simple, single-step technique for selecting and cloning hybridomas for the production of monoclonal antibodies.
He was awarded the Nobel Prize in Physiology or Medicine in 1984 with Georges Köhler, for developing the hybridoma technique for the production of monoclonal antibodies. This was the discovery that ...
In 1978 he spent a year with Dr. Klaus Rajewsky in Cologne, West Germany where he learned hybridoma technology and also isolated one of the most widely used fusing plasmacytomas. He has since ...
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