“This idea evolved into the notion that if you have two primers that are interacting with recombinase proteins and they move in opposite directions along a double-stranded piece of DNA, wouldn’t you ...

A team of scientists has created a new guide to advance standards for collecting and cataloging DNA from marine species, ...

The replication and amplification of DNA, using dNTPs, is a cornerstone of modern molecular biology, and has been so for more than 50 years. The methods have helped researchers reveal countless ...

qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by ...

Biocartis Announces Launch of the Brand-New Idylla™ POLE-POLD1 Mutation Assay . Mechelen, Belgium, 02 April 2025 – Biocartis NV (“Bioca ...

Figure 1: Amplification of complex gene libraries by conventional PCR and emulsion PCR. Figure 2: Ethidium bromide–stained agarose gels reveal the absence of size competition and chimeric ...

This is done without the need for nucleic acid amplification. In CRISPR/Cas-based diagnostic tests, guide RNAs bind pathogen DNA or RNA, triggering Cas enzymes to become active and cleave the ...

Their CRISPR-Cascade reaction utilizes two ribonucleoprotein complexes, T1 and T2, to achieve amplification-free detection of pathogenic DNA. Upon target recognition, Cas12a in the T1 complex ...